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Doç.Dr.Jale Çoban CLINICAL ENZYMOLOGY. GENERAL PROPERTIES OF ENZYMES Enzyme Specificity Enzyme Concentration and Activity Molecular Basis for Enzyme Catalysis.

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1 Doç.Dr.Jale Çoban CLINICAL ENZYMOLOGY

2 GENERAL PROPERTIES OF ENZYMES Enzyme Specificity Enzyme Concentration and Activity Molecular Basis for Enzyme Catalysis Nomenclature of Enzymes Isozymes Enzyme Cofactors Factors Affecting Plasma Enzyme Activities Factors Affecting the Lifetime of Enzymes in Blood

3 All enzymes are proteins and function as catalysts ( accelerate the rates of chemical reactions in the body but do not become chemically altered themselves in these reactions). Enzymes lower the activation energies of the chemical reactions that they catalyze, so as to cause greatly enhanced rates of reaction.

4 Enzymes interact very specifically with their substrates (do not interact with any molecules other than their substrates). The lock and key model of substrate binding to the ezyme active site.

5 NOMENCLATURE OF ENZYMES Each enzyme has two names: a practical or trivial name, and a systematic name. The latter consists of a unique numeric code designation and the nature of the catalytic reaction, as follows: Enzymes are named by citing the name of the substrate molecule and by following that with the suffix, -ase; sometimes the name also includes a designation of the type of reaction catalyzed.

6 The concentrations and/or activities of different enzymes in blood give vital information about the functioning of specific tissues (enzymes can serve as biomarkers for diseases in specific tissues).

7 ISOZYMES differ in sequence similar enzymatic activity Many enzymes have isozymes - polypeptide chains that differ in sequence but have similar enzymatic activity. Different isoenzymes are found in specific organs or tissues; determination of the type of isoenzyme present can then be of use in identifying the damaged tissue and releasing the enzyme. Isoenzymes are those that are composed of varying combinations of subunits. Common examples are creatine kinase ( CK ), a dimer of muscle (M) and brain (B) subunits, and lactate dehydrogenase ( LDH ), a tetramer of heart (H) and (M) subunits. Isoenzymes may have the same protein component but may differ based on modifications made by the cell of origin.

8 ENZYME COFACTORS Two thirds of all enzymes contain cofactors. They are low molecular weight organic molecules and inorganic ions. The combination of cofactor plus the protein portion (apoenzyme), forms the complete catalytic entity and is known as the holoenzyme. Organic cofactors are bound covalently or noncovalently to the apoenzyme. Covalently bound cofactors are sometimes referred to as prosthetic groups. Cofactors are observed in oxidation–reduction, group transfer. Inorganic Cofactors for Enzymes

9 Tissue localization Relative Amounts of Enzymes in Various Organs (Relative to Serum) serum Red cells liver heart Sceletal muscle AST 1 x 15 x 7000 x 8000 x4500 x ALT 1 x 7 x 3000 x 400 x1200 x 300 LDH 1 x 500 x 7000 x 600 x500 x 700 CPK x x10 x kidney

10 Molecular localization 1.membraner - ALP, GGT, 5’NU 2.Sitoplazmik - AST, ALT, LDH, CK 3.Mitokondriyal - AST, CK 4.lizozomal - ACP 5.Nükleer- Polimerazlar 6.mikrozomal - GGT

11 Hücre membranı, enzimlerin hücre dışına çıkmasını engelleyerek sellüler enzimlerin normalde hücre içinde kalmasını sağlar, sellüler enzimlerin ancak çok az bir kısmı hücre dışına çıkar ve dolayısıyla serumda bulunabilir.

12 Hücre membranının bütünlüğü, hücrenin enerji üretimine bağımlıdır. Enerji üretimi için gerekli oksijen girişinin kısıtlanması gibi enerji üretimini bozan her türlü etken, hücre membranının bozulmasını artırır. Bu gibi durumlarda, membran hücresel bileşenlerini sızdırır ve eğer hücresel hasar dönüşümsüz hale gelirse hücre ölür.

13 Enzimlerin hücrelerden serbest kalma hızının arttığı iki temel durum:  hücre harabiyeti (nekroz) ve  enflamasyondur.

14 NEKROZ Nekroz, hücre membranı ve organel membranlarının bütünlüğünün bozulmasıyla hücre içeriğinin hücre dışına boşalmasıdır. Bu durumda hem sitozolik hem mitokondriyal enzimler hücre dışına çıkar ve dolayısıyla serumda hem sitozolik hem mitokondriyal enzimlerin aktivitelerinin arttığı saptanır. Serum enzim düzeyi artışının saptanması suretiyle hücre harabiyetinin derecesi ve yaygınlığı hakkında bilgi edinilebilir. Hücre harabiyeti ve ölümüne neden olan faktörler şunlardır: Hipoksi, kimyasal ajanlar ve ilaçlar, fiziksel ajanlar, genetik bozukluklar, beslenme bozuklukları, enfeksiyöz ajanlar, immün bozukluklar.

15 Anemi ve kardiyorespiratuvar yetmezlik hallerinde hipoksi gelişebilir.

16 Kurşun, cıva, alkol, tütün gibi kimyasal ajanlar ve antiepileptikler gibi ilaçlar, hücre harabiyeti veya hücre ölümüne sonuçta da serumda hem sitozolik hem mitokondriyal enzimlerin aktivitelerinin artışına neden olabilir.

17 Travmalar, aşırı sıcak veya soğuk, radyasyon, hücre harabiyeti veya hücre ölümüne sonuçta da serumda hem sitozolik hem mitokondriyal enzimlerin aktivitelerinin artışına neden olan önemli fiziksel ajanlardır.

18 Vitamin ve mineral eksiklikleri ile sonuçlanan beslenme bozuklukları, serumda hem sitozolik hem mitokondriyal enzimlerin aktivitelerinin artışına neden olabilir.

19 Enflamasyon, kan damarları duvarı ve çevre dokularda hücre membranı geçirgenliğinin artışı ile ilgili reversibl bir olaydır, kimyasal veya biyolojik bir uyarı sonucu gelişir. Enflamasyonda organel membranları etkilenmediğinden serumda sadece sitozolik enzimlerin aktivitesi artar, mitokondriyal enzimlerin aktivitesinde önemli artış olmaz.

20 FACTORS AFFECTING PLASMA ENZYME ACTIVITIES

21 The most common cause for increased plasma enzymes is death of enzyme-containing cells. Direct damage to the cell membrane by viruses or by chemical substances Hypoxia, anoxia and ischemia of the tissues

22 FACTORS AFFECTING PLASMA ENZYME ACTIVITIES Enzymes are also released in the process of normal cell turnover. Increased synthesis of enzymes by cells also leads to increased plasma enzyme levels. İncreased activity of osteoblasts, plasma levels of the bone isoenzyme of ALP increase. İncrease in muscle-related enzymes seen with increased exercise. Many drugs that stimulate microsomal enzymes, including ethanol and antiepileptic agents, lead to increased plasma γ- glutamyl transferase (GGT).

23 FACTORS AFFECTING PLASMA ENZYME ACTIVITIES Release of enzyme from cells occurs without cell death or increased synthesis. Ethanol causes expression of the mitochondrial isoenzyme of AST( mAST ) on the surface of hepatocytes and increased plasma levels. Ischemia of myocardial cells leads to loss of glycogen phosphorylase BB isoenzyme and the MB isozyme of CK into plasma. Ingestion of food leads to release of intestinal ALP into plasma. A number of liver enzymes (ALP, GGT, leucine aminopeptidase, 5′- nucleotidase are bound to the canalicular surface of the hepatocyte. Increased concentrations of bile salts with canalicular obstruction may release fragments of membrane with enzyme attached into the circulation.

24 FACTORS AFFECTING PLASMA ENZYME ACTIVITIES İncreased plasma enzyme levels may be due to decreased clearance of enzymes from the circulation. Some smaller enzymes, such as amylase and lipase, are partially cleared by glomerular filtration; renal failure increases their plasma levels. For many enzymes, autoantibodies against one or more isoenzymes may cause the development of enzyme– antibody complexes (often termed macroenzymes ), which result in enzyme half-lives similar to the 3-week half-life of immunoglobulin G. for example: against the intestinal isoenzyme of ALP in persons with bacterial infection

25 INFORMATION AVAILABLE FROM ENZYME MEASUREMENTS Magnitude of damage (i.e. how many cells damaged?) Severity of damage (i.e. how badly is each cell damaged?) Stage of illness and prognosis Which tissue is damaged

26 FACTORS AFFECTING THE LIFETIME OF ENZYMES IN BLOOD With cell death, defects in cellular membranes enlarge gradually over time; thus, smaller cytoplasmic enzymes will leak from damaged cells sooner than larger ones. For example:  with myocardial injury, CK and AST are smaller than LDH and appear in plasma sooner.  Some enzymes are not cytoplasmic, but may be within mitochondria (isoenzymes of CK and AST) or bound to plasma membranes (such as ALP and GGT); cell death typically does not lead to release of such enzymes.

27 DETERMİNATİON OF TOTAL ENZYME CONCENTRATİONS İN SERUM AND OTHER BODY FLUİDS

28 (IU) DETERMINATION OF ENZYME ACTIVITY

29 In principle, in the measurement of enzyme activity, either the rate of disappearance of substrate or the rate of appearance of product can be measured. In general, it is easier to measure small increases in product than to measure small decreases in a relatively high concentration of substrate often needed to achieve saturation.

30 KLİNİK ENZİMOLOJİDE ENZİM ÖLÇÜMLERİ 1) Reaksiyon hızlarının ölçülmesi 2) Enzim kütlesinin ölçümü 3) İzoenzimlerin ve izoformların ölçümü

31 Measure enzyme activity in a sample, it is most common to use substrates at saturating concentrations where zero-order kinetics with respect to [S] occur. Under these conditions, the reaction rate is directly proportional to the total amount of enzyme present.

32 REAKSIYON HıZLARıNıN ÖLÇÜLMESI Enzim katalizli reaksiyonların hızı, sistemde mevcut olan aktif enzimin miktarı ile doğru orantılıdır. Reaksiyon hızının belirlenmesi, tanımlanmış bir zaman aralığında ortaya çıkan miktar değişiminin kinetik ölçümü suretiyle olur. Reaksiyon hızının ölçülmesinde sabit zaman ve sürekli izleme yöntemleri kullanılır. Sabit zamanlı reaksiyonda, enzim tarafından dönüştürülen ürün miktarı, sabit zaman aralığının sonunda reaksiyon durdurularak ölçülür. Sürekli izleme yönteminde, reaksiyonun ilerleyişi sürekli olarak izlenir. Reaksiyon hızının ölçümü, aktivite birimi ile ifade edilir. Klinik enzimolojide enzim aktivitesi genelde belli bir hacimde (100 mL’deki, L serumda, 1 mL eritrositteki gibi) verilir. Reaksiyon hızı pH, çözelti tipi, sıcaklık, substratın doğası, iyonik güç, aktivatör konsantrasyonu ve diğer değişkenler gibi deneysel parametrelere dayandığından, bu gibi parametreler birimin tanımlanmasında belirtilmelidir.

33 Sabit zamanlı reaksiyonda, enzim tarafından dönüştürülen ürün miktarı, sabit zaman aralığının sonunda reaksiyon durdurularak ölçülür. Sürekli izleme yönteminde, reaksiyonun ilerleyişi sürekli olarak izlenir.

34 Reaksiyon hızının ölçümü, aktivite birimi ile ifade edilir. Klinik enzimolojide enzim aktivitesi genelde belli bir hacimde (100 mL’deki, L serumda, 1 mL eritrositteki gibi) verilir. Reaksiyon hızı pH, çözelti tipi, sıcaklık, substratın doğası, iyonik güç, aktivatör konsantrasyonu ve diğer değişkenler gibi deneysel parametrelere dayandığından, bu gibi parametreler birimin tanımlanmasında belirtilmelidir.

35 Enzim aktivitesinin tanımlanmasını standardize etmek için IUB Enzim Komitesi, enzim aktivite biriminin dakikada 1 mikromolar substratı ürüne dönüştüren enzim miktarı olarak tanımlanmasını ve bu birimin uluslararası ünite (IU, U) olmasını önermiştir. Katalitik konsantrasyon, sayısal olarak daha uygun bir değer olduğu için, U/L veya mU/L birimleriyle ifade edilir.

36 Eğer bir enzim reaksiyonu ölçüm sistemindeki bazı bileşenlerin görünen veya ultraviyole spektrumda absorbans özelliklerinde değişiklikler ile beraber gerçekleşiyorsa bu durum spektrofotometrik olarak saptanır.

37 SPECTROPHOTOMETRIC ASSAYS

38 DETERMINATION OF ENZYME ACTIVITY Photometrically measured change in the absorbance of an indicator per unit of time is used as a measure of the rate of reaction. Photometrically measured change in the absorbance of an indicator per unit of time is used as a measure of the rate of reaction. This is proportional to the enzyme activity, provided that the enzyme is working at maximum rate (with sufficient substrate and coenzyme) ‏ This is proportional to the enzyme activity, provided that the enzyme is working at maximum rate (with sufficient substrate and coenzyme) ‏

39 DETERMINATION OF ENZYME ACTIVITY The coenzymes NAD and NADH are often used as indicators (optical test), less often the absorbance of the substrate or the reaction product (colorimetrik test). The coenzymes NAD and NADH are often used as indicators (optical test), less often the absorbance of the substrate or the reaction product (colorimetrik test). The activity is expressed in kinetic unit: The activity is expressed in kinetic unit: International unit (IU) is the quantity of enzyme which catalyzes one micromole (  mol) of substrate per minute. (Enzyme activity is expressed in IU/L, or in mU/L Lactate LDH Pyruvate Lactate LDH Pyruvate NAD + NADH

40 For example, the NADH/NAD+ molecule is often used in enzymatic oxidation/reduction reactions. During these reactions NADH is often oxidized to NAD+, or NAD+ is reduced to NADH. NADH absorbs light at 340 nm, however NAD+ does not hold that property. A spectrophotometer can be used to measure the change in absorbance of 340 nm light, thus indicating a change in amount of NADH.

41 Bu reaksiyonlara önemli örnekler, yükseltgenme ve indirgenmelerde NADH ve NADPH koenzimlerinin 339 (340) nm’deki absorbans değişimleri izlenerek dehidrogenaz aktivitesinin belirlenmesi Lactate LDH Pyruvate NAD + NADH

42 1 enzim ünitesi, optimal şartlarda (optimal ısı ve optimal pH) 1 mikromol substratı 1 dakikada ürüne dönüştüren enzim aktivitesidir; buna internasyonal ünite adı verilir. Günümüzde enzim ölçüm birimi olarak genelde bu ünite kullanılır; IU veya kısaca U şeklinde kısaltılır. 1 saniyede 1 mol substratı ürüne dönüştüren enzim aktivitesine 1 katal veya 1 SI ünitesi denir

43 KAN ENZİMLERİNİN AKTİVİTE TAYİNLERİNDE DİKKAT EDİLECEKLER

44 Kan enzim aktiviteleri tayini için ideal örnek serumdur, plazmadaki antikoagulanlar enzimatik aktiviteyi düşürürler. Enzim aktivitesi tayini yapılacak kan, antikoagulansız tüpe (düz tüp) alınmalıdır.

45 Kan alırken hemolizden kaçınmalıdır. Çünkü eritrositler, enzim bakımından çok zengindirler, hemoliz sonucunda eritrositlerdeki enzimler seruma geçerler ve sonuçta enzim aktivitesinde yanlış olarak yüksek değerler bulunur. Ayrıca seruma geçen hemoglobinin kırmızı rengi de interferans etkisiyle ölçüm hatalarına neden olur.

46 Serum için alınan kan, pıhtılaşmasından hemen sonra santrifüj edilerek serum ayrılmalıdır. Serumun hemen ayrılmaması halinde hücresel elemanlardaki enzimler seruma sızabilir ve enzim aktivitesinde yanlış olarak yüksek değerler bulunur.

47 Enzim tayinlerinde günlük taze kan kullanılması en iyisidir. Çünkü enzimlerin aktiviteleri zamanla azalır. Bazı enzimlerin aktivitelerinde +4 o C de 1-2 gün büyük bir kayıp olmadığı halde bazı enzimler oda sıcaklığında birkaç saat içinde inaktive olurlar.

48 SPECIFIC ENZYMES

49 DIAGNOSTIC ENZYMES Alanine aminotransferase (ALT) ‏ Aspartate aminotransferase (AST) ‏ Alkaline phosphatase (ALP) ‏ Gamma glutamyl transferase (GGT) ‏ Lactate dehydrogenase (LDH) ‏ α-Amilase Lipase Creatine kinase (CPK), Creatine kinase MB (CK-MB) ‏ Acid phosphatase 5′-Nucleotidase Leucine aminopeptidase Aldolase G-6-PD ChE

50 Bone enzymes  ALP Muscle enzymes  CK, CK-MB  LDH  Aldolaz  AST Malignant diseases enzymes  ACP  ALP  GGT  LDH Haematologyc enzymes  G-6-PDH  Piruvat kinase Liver enzymes  ALT  AST  LDH  GGT  ALP  5’nükleotidaz Pancreatic enzymes  Alfa-amilaz  Lipaz

51 LIVER ENZYMES

52 HEPATOCELLULAR ENZYMES As metabolically complex cells, hepatocytes contain high levels of a number of enzymes. With liver injury, these enzymes may leak into plasma and can be useful for diagnosis and monitoring of liver injury. Within the hepatocyte, the commonly measured enzymes are found in specific locations ; the type of liver injury will determine the pattern of enzyme change.

53 LOCATION OF HEPATOCELLULAR ENZYMES  ALT and the cytoplasmic isoenzyme of aspartate aminotransferase ( ASTc ) are found primarily in the cytosol. With membrane injury as in viral or chemically induced hepatitis, raising plasma AST and ALT activities.  Mitochondrial aspartate aminotransferase (ASTm) is released primarily with mitochondrial injury, as caused by ethanol as in alcoholic hepatitis.

54 LOCATION OF HEPATOCELLULAR ENZYMES  ALP and GGT are found primarily on the canalicular surface of the hepatocyte. Bile acids accumulate in cholestasis and dissolve membrane fragments, releasing bound enzymes into plasma. are increased by obstructive processes.  GGT is also found in the microsomes, microsomal enzyme-inducing drugs, such as etanol and phenobarbital, can also increase GGT synthesis and raise plasma GGT activity.

55 aspartate aminotransferase (AST) and alanine aminotransferase (ALT) AMINOTRANSFERASES (TRANSAMINASES)

56 These enzymes catalyze reversibly the transfer of an amino group of AST or ALT to α-ketoglutarate to yield glutamate plus the corresponding ketoacid of the starting amino acid (i.e., oxaloacetate or pyruvate, respectively). Both enzymes require pyridoxal phosphate (vitamin B 6 ) as a cofactor.

57 AST and ALT have respective blood half-lives of 17 and 47 hours, respectively, and have upper reference range limits of around 35 IU/L. AST is both intramitochondrial and extramitochondrial, but ALT is completely extramitochondrial. Mitochondrial AST isoenzyme has a half-life of 87 hours. AST is ubiquitously distributed in the body tissues, including the heart and muscle, whereas ALT is found primarily in the liver, although significant amounts are also present in the kidney.

58 Tissue localization Relative Amounts of Enzymes in Various Organs (Relative to Serum) erythocytesliverheart Sceletal muscle AST x 15 x 7000 x 8000 x4500 x ALT x 7 x 3000 x 400 x 1200 x 300 kidney

59 MECHANISMS OF ENZYME RELEASE FROM HEPATOCYTES Enzymes are released from hepatocytes as a result of injury to the cell membrane that directly causes extrusion of the cytosolic contents. Agents like ethanol cause release of mAST from hepatocytes and its expression on cell surfaces. Accumulation of bile salts with canalicular obstruction causes release of membrane fragments with attached canalicular enzymes. Increased synthesis of GGT, and to a lesser extent alkaline phosphatase, can occur with medications that induce microsomal enzyme synthesis, notably ethanol, phenytoin, and carbamazepine.

60 ASSAYS FOR AST AND ALT In this reaction, nicotinamide adenine dinucleotide (NADH) is converted to NAD that is measured by a decrease in absorbance at 340 nm. As noted earlier, it is vital that pyridoxal phosphate be present in sufficient quantity to allow these reactions to proceed. TRIS,L-Alanin,LDH (mikrobiyal),Piridoksal fosfat L,NADH,2-Oksoglutarat pH (37 °C) L-Alanin + 2-oksoglutarat ALT piruvat + L-glutamat Piruvat + NADH + H+ LDH L-laktat + NAD+

61 AST L-Aspartat + 2-oksoglutarat oksaloasetat + L-glutamat MDH Oksaloasetat + NADH + H+ L-malat + NAD+

62 ALT Stabilite: °C’de 3 gün 2-8 °C’de 7 gün -70 °C’de > 7 gün Sitrat ve florür enzim aktivitesini inhibe eder. AST °C’de 7 gün 2-8 °C’de 7 gün

63 Normally not affected2-5 x normal Acute Pancreatitis Normally not affectedUp to 3 x normal Pulmonary emboli Normal to slightly increase2-5 x normal Skeletal muscle injury Variable increases, up to 2 x normal Cirrhosis Up to 5 x normal Obstructive jaundice, cholangitis Up to 20 x normal Toxic Hepatitis x normal Viral Hepatitis 5-10 x normal>10 x normal Heart failure with liver congestion Normal to slightly increase4-5 x normal Myocarditis Normal to slightly increase4-5 x normal Pericarditis Normal to slightly increase4-5 x normal Congestive Heart Failure Normal to slightly increase in uncomplicated infarcts 4-5 x normal 4-5 x normal ; >10-15 x normal ~ fatal infarcts Myocardial Infarction Increased Activity Low normal to below normal; corrected by some methods Vitamin B 6 deficiency Not applicableSuppression of activity Uremia Decreased Activity ALTAST Condition Clinical Causes of Increased or Decreased Serum AST and ALT Activity

64 ASPARTATE AMINOTRANSFERASE (AST) Interfering factors: Increased with exercise, antihypertensive drugs, cholinergic agents, coumadin, digitalis. erythromycin, oral contraceptives, opiates, salicylates. False decrease in uremia, severe long-standing liver disease, diabetic ketoacidosis

65 ALANINE AMINOTRANSFERASE (ALT) Interfering factors: Increased large number of IM injections Acetaminophen, ampicillin, codeine, nafcillin, oral contraceptives, salicylates, tetracycline, other drugs

66 These enzymes are located predominantly on the canalicular membrane of the hepatocyte. alkaline phosphatase ( ALP ) γ-glutamyl transferase ( GGT ) and 5′-nucleotidase. In contrast to cytoplasmic enzyme activities, canalicular enzyme activities within hepatocytes are typically quite low; focal hepatocyte injury seldom causes significant increases in canalicular enzyme levels. ENZYMES PRIMARILY REFLECTING CANALICULAR INJURY

67 (ALP) ALKALINE PHOSPHATASE

68 Alkaline phosphatases represent a family of enzymes coded for by different genes. ALP is present in a number of tissues, including liver, bone, kidney, intestine, and placenta, each of which contains distinct isozymes that can be separated from one another by electrophoresis. Hepatocytes produce ALP in the liver, where it is found attached to the canalicular surface of the cells. Osteoblasts produce bone ALP, which appears to be involved in cleavage of pyrophosphate, an inhibitor of bone mineralization. Intestinal epithelial cells produce intestinal ALP, which is released into the intestine following ingestion of fatty foods. Plasental ALP

69 With liver injury, ALP synthesis increases, but bile acids dissolve fragments of canalicular cell membranes with attached enzymes (including ALP, GGT, leucine aminopeptidase, and 5′-NT. In normal serum, a single form (of liver or bone origin) of ALP is typically seen The intestinal isoenzyme of ALP is released in large amounts into duodenal fluid, and large amounts enter lymphatic fluid, draining the intestinal tract following a meal MECHANISMS FOR RELEASE OF ALP FROM CELLS

70 The higher values in children are due to the bone isoenzyme. Pregnancy causes a two- to threefold increase in ALP, mainly due to the placental isoenzyme, but also because of an increase in bone isoenzyme. REFERENCE RANGES FOR ALP ARE HIGHLY DEPENDENT ON AGE AND GENDER

71 PREANALYTIC VARIATION High body mass index is associated with a 10% average increase in ALP. Oral contraceptives decrease ALP by an average of 20%. Fibric acid derivatives decrease total ALP by 25% and the liver isoenzyme by 40%. Antiepileptic agents commonly cause increased total ALP ( mainly because of increases in the liver isoenzyme). Smoking causes an average 10% increase in total ALP as the result of pulmonary production of placental-like ALP.

72 MEASUREMENT ALP activity is usually measured using p -nitrophenyl phosphate as the substrate at alkaline pH. Hydrolysis of this phosphate ester yields inorganic phosphate plus the highly colored and easily measured para -nitrophenoxide anion. ALP p-nitrofenil fosfat + H2O fosfat + p-nitrofenol Zn is a component of the enzyme, and Mg and other cations activate the enzyme. Chelators present in collection tubes (such as EDTA, citrate, and oxalate) falsely lower ALP activity; in the case of EDTA, activity is often too low to measure.

73 ALP The activity of the enzyme increases slowly on storage because of loss of inhibitors, but specimens are relatively stable at : °C - 7 d 2-8 °C - 7 d (-15)-(-25) °C - 2 m

74 CAUSES OF ABNORMAL RESULTS The most common causes of increased ALP are liver and bone disease. Disorders causing cholestasis more frequently cause elevation of ALP than do hepatocellular disorders. In obstructive cholestasis, ALP most commonly rises to twice the upper limit of normal or greater, roughly paralleling the rate of rise in serum bilirubin. If obstruction is partial, ALP usually increases as much as with complete obstruction, often out of proportion to the increase in conjugated bilirubin.

75 Passive congestion of the liver can occasionally result in moderate ALP elevations, more so than abnormal bilirubin levels. ALP is also moderately elevated in most instances of jaundice resulting from hepatic injury. When the resulting cholestasis is relieved, serum ALP levels fall to normal more slowly than bilirubin.

76 CAUSES OF ABNORMAL RESULTS Increased osteoblastic activity in Paget's disease, osteosarcoma, tumor metastatic to bone, and metabolic bone disease are the most common causes of elevated bone isoenzyme. Patients will have elevations of both bone and liver isoenzymes, especially in metastatic carcinoma. Increases in intestinal ALP may occur in patients with intestinal infarction, inflammation, and ulceration.

77 CAUSES OF ABNORMAL RESULTS Increases in placenta-like isoenzymes, such as Regan and Nagao, are commonly found in patients with malignancies (ovary, cervix, lung, breast, colon, pancreas) and are due to ectopic production by the neoplasm. Decreased ALP can also occur in zinc deficiency because zinc is a necessary cofactor for ALP activity, as well as other conditions. Placental alkaline phosphatase (PLAP) is a useful tumor marker in serum and cerebrospinal fluid (CSF) for most germ cell tumors.

78 (GGT) GAMMA-GLUTAMYL TRANSFERASE

79 This enzyme regulates the transport of amino acids across cell membranes by catalyzing the transfer of a glutamyl group from glutathione to a free amino acid.

80

81 Not elevated with pregnancy, bone disease, childhood or adolescence. Not elevated with pregnancy, bone disease, childhood or adolescence. Normal GGT + Elevated ALP is suggestive of bone disease. Normal GGT + Elevated ALP is suggestive of bone disease. Elevated GGT + Elevated ALP is suggestive of liver or bile-duct disease. Elevated GGT + Elevated ALP is suggestive of liver or bile-duct disease.

82 Its major use is to discriminate the source of elevated ALP (i.e., if ALP is elevated and GGT is correspondingly elevated, then the source of the elevated ALP is most likely the biliary tract). The highest values, often greater than 10 times the upper limit of normal, may be found in chronic cholestasis due to primary biliary cirrhosis or sclerosing cholangitis.

83 This enzyme is also elevated in about 60%–70% of those who chronically abuse alcohol, with a rough correlation between amount of alcohol intake and GGT activity. Levels often decline slowly with abstention from alcohol and remain elevated for at least 1 month after abstinence begins. GGT has a half-life of 10 days, but, in recovery from alcohol abuse, the half-life may be as long as 28 days. It tends to be higher in obstructive disorders and with space-occupying lesions in the liver than with hepatocyte injury.

84 Serum levels of GGT differ from those of ALP during pregnancy, in which GGT remains normal even during cholestasis in pregnancy. GGT is often increased in alcoholics even without liver disease; in some obese people; and in the presence of high concentrations of therapeutic drugs, such as acetaminophen and phenytoin and carbamazepine (increased up to five times the reference limits)

85 MEASUREMENT Most assays for GGT utilize the substrate γ-glutamyl–p- nitroanilide. In the reaction catalyzed by GGT, p-nitroaniline is liberated and is chromogenic, enabling this colored product to be measured spectrophotometrically. GGT L-γ-glutamil-3-karboksi-4-nitroanilit + glisilglisin L-γ-glutamil-glisilglisin + 5-amino-2-nitrobenzoat

86 OTHER ENZYMES 5′-Nucleotidase activity is increased in cholestatic disorders with virtually no increase in activity in patients with bone disease. Measurement of 5′-nucleotidase can corroborate the elevation of ALP from a hepatic source. Leucine aminopeptidase (LAP), can be used for the same purpose but virtually never are. Isocitrate dehydrogenase activity is elevated in hepatocellular injury and parallel ALT and AST..

87 LACTATE DEHYDROGENASE

88 This cytosolic glycolytic enzyme catalyzes the reversible oxidation of lactate to pyruvate. LDH Lactate Pyruvate NADNADH NAD + NADH

89 Tissue localization LDH is present in varying amounts in the e cytoplasm of all cell in the body.Elevations of total LDH activity are therefore found in many pathological conditions. erythocytes liver heart Sceletal muscle LDH x 500 x 7000 x 600 x500 x 700 kidney

90 Each LDH molecule consists of 4 subunits: Heart (H) and Muscle (M) type. İn the tissues the H and M type are combined into five izoenzymes. The H type predominates in tissues with high O2 consumption, the M type in tissues with high glycolytic activity. LDH 1 Heart LDH 1 ; HHHH; H4 Heart, erythrocytes,kidney LDH 2 LDH 2 ; HHHM; H3M LDH 3 ; HHMM; H2M2 Spleen, lung, lymph nodes,platelets LDH 4 LDH 4 ; HMMM; H3M Liver, skeletal muscle Liver, skeletal muscle LDH 5 LDH 5 ; MMMM; M4

91 The total LDH measurable in serum consists of the activities of the five isoenzymes LDH1 to LDH 5. The total LDH measurable in serum consists of the activities of the five isoenzymes LDH1 to LDH 5. However, if the total LDH is elevated, quantitative differention of the isoenzymes can provide diagnostically useful organ-related information. However, if the total LDH is elevated, quantitative differention of the isoenzymes can provide diagnostically useful organ-related information.

92 The upper reference range limit for total LDH activity in serum is around 150 IU/L. LDH values are highest in newborns and infants; values do not change with age in adults, and there is no gender difference. Serum LDH levels become elevated in hepatitis; often, these increases are transient and return to normal by the time of clinical presentation because LDH isozymes originating in liver (LD 4 and LD 5 ) have relatively low activity in hepatocytes relative to plasma (about 500 times) and a half-life of approximately 4–6 hours.

93 PREANALYTIC VARIATION Even trace to slight hemolysis invalidates LD and LD isoenzyme analyses. Contact with the clot increases LD, and physical agitation of specimens, as occurs in most pneumatic tube systems, tends to cause some hemolysis and increased LD. Exercise has little effect on LD or its isoenzymes. Extreme exercise can cause LD 1 to become greater than LD 2. Total LDH increases transiently after blood transfusion but returns to baseline within 24 hours. Delayed separation of red cells from serum does not affect LD values for 1 to 2 days. Few drugs directly affect LDH activity, but granulocyte- macrophage colony-stimulating factor appears to increase LDH in parallel to the increase in white blood cell. (WBC) count.

94 MEASUREMENT LD activity can be measured using either the forward (lactate-to-pyruvate) or the reverse (pyruvate-to-lactate) direction of the reaction. A vast majority of laboratories use the forward reaction. The reverse (pyruvate-to-lactate) reaction is used in a few laboratories currently because of faster reaction kinetics, the less costly cofactor (NADH) needed, and the smaller specimen volume requirement. Disadvantages of the pyruvate-to-lactate reaction include early loss of linearity of reaction kinetics, the effect of potent LD inhibitors in some NADH preparations, and use of suboptimal concentrations of pyruvate because of substrate inhibition. Also, lactate is a more specific substrate for this enzyme; pyruvate is less specific and serves as a substrate for such enzymes as pyruvate dehydrogenase. Electrophoretic separation of LD isoenzymes is typically used when quantitation of different isoenzymes is required; agarose gel is most commonly used. Quantitation usually uses the forward reaction, allowing detection of fluorescent NADH or a reduced formazan dye in a colorimetric development step.

95 Serum LD is, on average, 30 IU/L higher than plasma LD, owing to release of LD from platelets. LD is not stable on storage at 4° C because of cold lability of LD 5. Specimens can be stored for up to 24 hours at room temperature with little change. Three days of storage at room temperature decreases total LD by about 20%. If specimens are frozen, LD 5 decreases significantly and the isoenzyme pattern shifts with an artifactual increase in LD 1 and decrease in LD 5. Serum should not be frozen for assay of LD or for its isoenzymes.

96 CAUSES OF ABNORMAL RESULTS LD is a highly nonspecific test; an abnormal value is not specific for damage to any particular organ. Relative amounts of LD, AST, and ALT (along with CK) may provide clues to the source of LD elevation. If LD is markedly elevated, but AST, ALT, and CK are normal or minimally increased, this suggests damage to cells such as red or white blood cells, kidney, lung, lymph nodes, or tumors. Increases in both CK and LD, with greater increases in AST than ALT, occur with cardiac or skeletal muscle injury. Increases in LD occur in liver disease, and LD can be elevated in hepatitis, although these increases by themselves are not specific to liver injury. In fulminant hepatic failure, the serum levels of ALT and LD both increase such that LD increases more rapidly initially. An ALT-LD index has been computed in these patients and was found to be a reliable predictor of survival in patients with this condition.

97 Serum elevations of LD and ALP frequently occur in space-occupying lesions of the liver; most cases are identified as metastatic carcinoma or primary hepatocellular carcinoma. In many conditions, such as shock and metastatic carcinoma, LD is increased because of damage to multiple organs, so that mixed patterns can be seen. CAUSES OF ABNORMAL RESULTS

98 Marked elevations of LD (>5 to 10 times normal) are seen in megaloblastic anemia, hemolytic anemias, malignancies (particularly lymphoma and leukemia), sepsis or other causes of shock, and cardiopulmonary arrest. In cases where the cause of elevated LD cannot be determined by other means, LD isoenzymes may be useful in determining the source of injury. In normal serum, the LD isoenzymes, in decreasing order of activity, are 2 > 1 > 3 > 4 > 5. In germ cell tumors (particularly seminoma and dysgerminoma), LD 1 is increased and can serve as a tumor marker.

99 CHANGES IN SERUM LDH AND LDH ISOENZYMES IN MI Myocardial Infarction (MI) ‏ Up to 10 x normal Up to 10 x normal A rise in LDH occurs 8-12 hours after the onset of pain. A rise in LDH occurs 8-12 hours after the onset of pain. Peak activity occurs between hours. Peak activity occurs between hours. Activity returns to normal by the 5th-6th day but may remain elevated up to 10 days. Activity returns to normal by the 5th-6th day but may remain elevated up to 10 days. The LDH-1 isoenzyme is increased: The LDH-1 isoenzyme is increased: LDH-1>LDH-2 pattern increases the specificity of the test.

100 ACUTE MI MARKERS

101 Variable increases, especially LDH-5, depending on the amount of trauma. Muscular trauma due to physical exercise or crush injuries >10 x normal. Cancer, Metastasis 2 x normal. LDH-5 predominates. Cirrhosis and Obstructive Jaundice Up to 5 x normal. LDH-5 predominates Viral Hepatitis Viral Hepatitis and Infectious Mononucleosis Increases in total LDH >5 x normal. Rises in the LDH-1 and LDH-2 isoenzymes. Megaloblastic Anemia Up to 10 x normal A rise in LDH occurs 8-12 hours after the onset of pain. Peak activity occurs between hours. Activity returns to normal by the 5 th -6 th day but may remain elevated up to 10 days. The LDH-1 isoenzyme is increased: a LDH-1>LDH-2 pattern increases the specificity of the test. Myocardial Infarction (MI) ‏ Relative or Absolute ChangeCondition Changes in Serum LDH and LDH Isoenzymes in Certain Disease States.

102 CK CREATINE PHOSPHOKINASE

103 Creatine Kinase (CK) is a cytoplasmic and mitochondrial enzyme that catalyzes both the formation of ATP and reversible phosphorylation of creatine with ATP as a donor phosphate group. CK requires metalloactivators, especially Mg ++ muscle tissue CK activity is especially important in muscle tissue, where it catalyzes the synthesis of Creatine Phosphate, a high energy storage molecule. Upon muscle contraction, the phosphate group is used to form ATP to provide immediate energy for the muscles.

104 TISSUE SOURCES skeletal muscle. The largest distribution of CK is found in skeletal muscle. Other tissue sources : brain, rectum, stomach, bladder, colon, uterus, prostate, small intestine and kidney. Negligible in the liver, placenta and thyroid tissue.

105 This enzyme has two major isoenzymes, M and B, each of which has the same activity. CK is composed of three types of dimers: CK- MM (mainly in skeletal muscle), CK- MB (found mainly in cardiac tissue), and CK- BB (found predominantly in brain and intestine).

106 The major CK isoenzyme in the serum of healthy subjects is, where it represents % of total CK activity. CK-MB is found in concentrations of less than %6 normally, whereas CK-BB is rarely detected. The major CK isoenzyme in the serum of healthy subjects is CK-MM, where it represents % of total CK activity. CK-MB is found in concentrations of less than %6 normally, whereas CK-BB is rarely detected.

107 Skeletal muscle injury can occur from a variety of sources. Direct trauma, as occurs with physical injury (including contact sports), surgery, strenuous exercise, and intramuscular injection, are common causes of mild elevations of CK (up to about five to six times reference limits). In these situations, CK typically increases rapidly and then falls quickly, returning to baseline with a half-life of approximately 24 hours.

108 An important clinical disorder associated with acute muscle injury is neuroleptic malignant syndrome, a rare complication of treatment with phenothiazines or other psychotropic agents. Typically, affected individuals present with muscle rigidity, fever, and elevated WBC counts; CK is considered a diagnostic test for this disorder. Prompt recognition and treatment are necessary to prevent death from this syndrome; discontinuation of medications and use of the muscle-stabilizing agent dantrolene are the cornerstones of treatment.

109 Chronic damage to muscle causes more persistent elevation of CK. Common causes of chronic muscle injury include medications (particularly 3-hydroxy-3-methylglutaryl- coenzyme A reductase inhibitors and glucocorticoids), congenital myopathies (such as Duchenne's muscular dystrophy), inflammatory disorders (such as polymyositis and dermatomyositis), hypothyroidism, and alcohol abuse. In chronic myopathies, CK-MB is often increased, reflecting its production by regenerating muscle.

110 With severe acute damage to muscle, a clinical picture termed rhabdomyolysis may develop. In such situations, CK-MB/total CK may be elevated above normal, in the absence of myocardial infarction (cardiac troponin levels are normal); this is commonly seen in patients with inflammatory muscle disease. In addition to normal cardiac troponin levels with elevated CK levels, two distinct isoforms (representing fast or slow skeletal fiber types) of serum skeletal troponin I may be identified.

111 ACETYLCHOLINESTERASE

112 AChE and PChE are two different enzymes produced by different tissues that are able to cleave acetylcholine, one of the body's major neurotransmitters. PChE is important in the cleavage of acetylcholine antagonists such as succinylcholine and mivacurium, muscle relaxants used during surgery.

113 Amylase and Lipase PANCREATIC ENZYMES

114 AMYLASE

115 Two isoenzymes for 1. Salivary 2. Pancreatic In normal conditions, about 60% of circulating amylase is salivary and 40% pancreatic.

116 Plasma specimens that have been anticoagulated with citrate or oxalate should be avoided for amylase determination because amylase is a calcium-containing enzyme. Heparinized plasma specimens do not interfere with the amylase assay. Diagnosis is confirmed by detection of elevated serum amylase threefold above normal. It peaks in 20–30 hours, often at 10–20 times the upper reference limit. Amylase returns to normal in 48–72 hours.

117 Elevated values persisting longer than this suggest continuing necrosis or possible pseudocyst formation. Serum amylase sensitivity is 72%, and specificity is 99%. Serum amylase has poor sensitivity for pancreatitis; it is not increased in about 20% of patients with pancreatitis.

118 Serum amylase increases nonspecifically in many acute abdominal conditions. In hyperlipidemic patients with pancreatitis, normal serum and urine amylase levels are frequently encountered. The spuriously normal levels are believed to be the result of suppression of amylase activity by triglyceride or by a circulating inhibitor in serum. Serum amylase levels do not correlate with cause or severity of pancreatitis. Amylase is also produced by the salivary glands.

119 The urine amylase activity rises promptly, often within several hours of the rise in serum activity, and may remain elevated after the serum level has returned to the normal range. Values greater than 1000 Somogyi units/hour are seen almost exclusively in patients with acute pancreatitis. In a majority of patients with acute pancreatitis, serum amylase activity is elevated, and a concomitant increase in urine amylase activity occurs.

120 Lower than normal serum amylase activity may be found in patients with chronic pancreatitis and has been seen in such diverse conditions as congestive heart failure, pregnancy (during the second and third trimesters), gastrointestinal (GI) cancer, bone fracture, and pleurisy.

121 Serum amylase may be elevated in patients with pancreatic carcinoma, but often too late to be diagnostically useful. Serum amylase activity may also be elevated in patients with cholecystitis, peptic ulcer, renal transplant, viral hepatitis, or ruptured ectopic pregnancy, or post gastrectomy.

122 LIPASE

123 The pancreas is the major and primary source of serum lipase. Lipase is not present in the salivary glands Serum lipase is more specific for the diagnosis of acute pancreatitis. Serum lipase increases in 4–8 hours and remains elevated for 8– 14 days. Increased lipase activity rarely lasts longer than 14 days; prolonged increases suggest a poor prognosis or the presence of a pancreatic cyst. Calcium is necessary for maximal lipase activity, but at higher concentrations it has an inhibitory effect. Similar to serum albumin, bile salts prevent the denaturation of lipase at the interface. Heavy metals and quinine inhibit lipase activity.

124 Lipase is filtered by the glomeruli owing to its low molecular weight; it is normally completely reabsorbed by the proximal tubules and is absent from normal urine. In patients with failure of renal tubular reabsorption caused by renal disorders, lipase is found in the urine. Urine lipase activity in the absence of pancreatic disease is inversely related to creatinine clearance.

125 Serum lipase is stable up to 1 week at room temperature and may be kept stable longer if it is refrigerated or frozen. The optimal reaction temperature is about 40° C. The optimal pH is 8.8. Serum is the specimen of choice for blood lipase assays. Icterus, lipemia, and hemolysis do not interfere with turbidimetric lipase assays.

126 Both serum lipase and amylase are useful in ruling out acute pancreatitis. Although determination of serum lipase has diagnostic advantages over serum amylase for acute pancreatitis, this value is not specific for acute pancreatitis. Serum lipase may also be elevated in patients with chronic pancreatitis, obstruction of the pancreatic duct, and nonpancreatic conditions, including renal disease, acute cholecystitis, intestinal obstruction or infarction, duodenal ulcer, and liver disease, as well as alcoholism and diabetic ketoacidosis, and in patients who have undergone ERCP. Patients with trauma to the abdomen have increases in both serum amylase and lipase. Elevation of serum lipase activity in patients with mumps strongly suggests significant pancreatic involvement by the disease.

127 ACID PHOSPHATASE

128 prostate Greatest concentrations occur in prostate, liver, spleen, and bone Lysosomal, prostatic, erythrocyte, macrophage, and osteoclastic ACP are five important types found in humans. Normally, concentrations in serum are low erythrocyte ACP The activity of erythrocyte ACP can be distinguished from that of the other ACP isoenzymes in that it is inhibited by 2% formaldehyde solution and 1 mM cupric sulfate solution. This is in contrast to the other isoenzymes, which are not inhibited by these agents. In addition, erythrocyte ACP is not inhibited by 20 mM tartrate solution, which does inhibit the other isoenzymes. Tartrate-resistant acid phosphatase Tartrate-resistant acid phosphatase (TRAP) is present in certain chronic leukemias and some lymphomas

129 ACID PHOSPHATASE prostatic adenocarcinoma. Usually the test is utilized for the measurement of prostatic serum ACP in the diagnosis or monitoring of prostatic adenocarcinoma.

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131

132 ALKALEN FOSFATAZ REFERANS ARALıKLARı

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139 METASTATIK MIDE CA

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148 AKUT PANKREATİT

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153 METASTATIK MIDE CA

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157 METASTATIK MEME CA

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159 METASTATIK PROSTAT CA

160 KOLESİSTİT

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162 METASTATIK AKCIĞER CA

163 SAFRA KESESİ TAŞI

164

165 PAROTITIS?

166 HEPATIT

167 WHICH DISEASE?

168 LIVER DISEASE ALT or AST Hepatocellular damage best assessed by ALT or AST. ALP or GGT Biliary damage best assessed by ALP or GGT. Pattern of enzyme changes will often show which is predominant.

169 CARDIAC DAMAGE Total CK more especially CK-MB Total CK and more especially CK-MB isoenzyme most useful markers of cardiac damage. CK-MB is the most specific marker of myocardial infarct, positive in greater than 98% of patients. ASTLDH (especially LD1 and LD2 isoenzymes) Other useful enzymes are AST and LDH (especially LD1 and LD2 isoenzymes) though these may be raised in liver diseases also.

170 PANCREAS DISEASE amylase Acute pancreatitis usually shows an elevation of amylase, which is small enough to pass through the glomerulus and appear in the urine.

171 BONE DISEASE Alkaline phosphatase Alkaline phosphatase is raised in bone diseases that have a rise in osteoblastic activity, e.g. Pagets disease, rickets, etc. There is a bone isoenzyme which may be differentiated from the other isoenzymes.

172 SKELETAL MUSCLE DISEASE Total CKCK-MM Total CK, and more especially CK-MM isoenzyme, most sensitive marker of skeletal muscle damage, e.g. muscular dystrophies, polymyositis, etc. AST and LDH may also be raised.

173 PROSTATE DISEASE Acid phosphatase (ACP) is generally raised if prostate carcinoma has spread outside the capsule. Useful enzyme to monitor effect of therapy. Nowadays replaced by prostate specific antigen (PSA).

174 WHICH ENZYME?

175 AST - Useful in liver disease, cardiac damage, skeletal muscle disease; may be raised with haemolysis. ALT - Useful in liver disease, especially hepatocellular, more specific than AST, but longer half life. ALP - Useful in liver disease, especially biliary disease and cholestasis; bone disease with osteoblastic involvement and carcinoma with osteoblastic metastases. GGT - Raised in virtually all liver diseases. Very sensitive (almost too much so), inducible enzyme, i.e. raised by certain drugs and alcohol. Associated with biliary system and may be used to differentiate between liver and bone ALP.

176 CK - has three isoenzyme forms: CK-MM found in high concentration in skeletal muscle CK-MB accounts for up to 20% of cardiac CK activity CK-BB found in neurological tissue Total CK Total CK is raised after MI or any myocardial damage (e.g. pacemaker insertion, cardiac surgery etc.) and skeletal muscle damage. CK-MB will differentiate between cardiac and skeletal muscle damage.

177 ALT L-alanin ile 2-oksoglutarat arasındaki reaksiyonu katalize eder. Oluşan piruvat L-laktat ve NAD+’nın oluştuğu laktat dehidrojenazın (LDH) katalize ettiği bir reaksiyonda NADH tarafından indirgenir. Piridoksal fosfat, amino transfer reaksiyonunda bir koenzim olarak görev yapar. Enzim aktivasyonunun tam olmasını sağlar. ALT L-Alanin + 2-oksoglutarat piruvat + L-glutamat LDH Piruvat + NADH + H+ L-laktat + NAD+ NADH yükseltgenmesinin hızı katalitik ALT aktivitesiyle doğru orantılıdır. Absorbanstaki azalma 340 nm’de ölçülerek tayin edilir.

178 Serum (hemolizsiz). Plazma (hemolizsiz): Li-heparin veya EDTA plazma. Diğer antikoagülanları kullanmayın. Tercih edilen örnek hemolize olmayan serumdur. Stabilite: °C’de 3 gün 2-8 °C’de 7 gün -70 °C’de > 7 gün

179 Numune içindeki AST oksaloasetat ve L-glutamatın oluşması için L- aspartat ile 2-oksoglutarat arasında bir amino grubunun transferini katalize eder. Oksaloasetat daha sonra NAD+’nın oluşması için malat dehidrojenaz (MDH) varlığında NADH ile reaksiyona girer. Piridoksal fosfat, amino transfer reaksiyonunda bir koenzim olarak görev yapar. Enzim aktivasyonunun tam olmasını sağlar. AST L-Aspartat + 2-oksoglutarat oksaloasetat + L-glutamat MDH Oksaloasetat + NADH + H+ L-malat + NAD+ NADH yükseltgenmesinin hızı katalitik AST aktivitesiyle doğru orantılıdır. Absorbanstaki azalma 340 nm’de ölçülerek tayin edilir.

180 Serum (hemolizsiz). Standart numune alma tüplerini kullanarak serum alın. Plazma (hemolizsiz): Li-heparin veya EDTA plazma. Diğer antikoagülanları kullanmayın. Tercih edilen örnek hemolize olmayan serumdur. Stabilite: °C’de 7 gün 2-8 °C’de 7 gün

181 ALP Standardize edilmiş bir yönteme göre kolorimetrik test p-nitrofenil fosfat, ortamda magnezyum ve çinko iyonları bulunduğunda, fosfataz aracılığıyla fosfat ve p-nitrofenole bölünür. ALP p-nitrofenil fosfat + H2O fosfat + p-nitrofenol Açığa çıkan p-nitrofenol katalitik ALP aktivesi ile doğrudan orantılıdır. Absorbanstaki artış 409 nm’de ölçülerek tayin edilir.

182 Serum Plazma: Heparin (Li-, Na-, NH4 +-) plazma. Stabilite: 15-25°C’de 7 gün 2-8°C’de 7 gün -20°C’de 2 ay

183 Sınırlamalar - etkileşim Kriter: Başlangıç değerinin ±%10’u içerisinde telafi edilir. Serum, plazma İkter Belirgin etkileşim yok. Hemoliz H indeksi 250 olana kadar belirgin etkileşim yok (yaklaşık hemoglobin konsantrasyonu: 155 μmol/L veya 250 mg/dL). Lipemi Belirgin etkileşim yok. Diğer Çok nadir durumlarda, gammopati, özellikle tip IgM (Waldenström makroglobulinemi) güvenilmez sonuçlara neden olabilir.


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